Utilizes a deformable mirror to correct for system-level nonlinearities and optical aberrations, enabling sharp imaging across the entire 50 mm² area.

The ULTRA system is specifically designed for complex, multi-regional brain studies.

Effective imaging up to 900 μm in the mouse brain, crucial for studying deep cortical layers.

Employs advanced scanning techniques to achieve a total imaged volume of over 45 mm³. 3. Key Applications in Neuroscience

Optimized for in vivo studies, involving anaesthetized animals to maintain consistency.

The system, likely utilizing a variant described as VAM_GS (possibly a reference to Volumetric Adaptive Microscopy/Gaussian Stacking or similar technique), focuses on maintaining high resolution across a large, non-flat area.

The system sequences through Zernike modes to compensate for aberrations, with optimization processes sometimes completing in minutes, tailored for high-speed scanning (e.g., 133 Hz for ROI).

Experiments have shown its capability to study both morphology (structural) and neuronal activity (functional) in mouse brains. 4. Implementation and Data Handling